Figure 3.

Schematic diagram of the structural organization of plasmid pM16/0594 from E. faecium isolate M16/0594, encoding the poxtA linezolid resistance gene resolved by hybrid assembly of paired-end Illumina MiSeq short reads with Oxford Nanopore Technologies long reads. The 4001 bp highly conserved region around poxtA, flanked by two IS1216E in parallel orientation, is highlighted in pink. Genes of interest and their orientation are represented by arrows as follows: red indicates antibiotic resistance genes, orange indicates ISs, blue indicates rep proteins, purple indicates other known proteins and yellow indicates hypothetical proteins. The plasmid size is labelled (bp). PRE, plasmid recombination enzyme; fic, cell filamentation protein; HyPro, hypothetical protein.