Figure 3.
Auphen Inhibits P. vivax Schizonts and Hypnozoites
(A) Table with EC50 values for auphen inhibition of P. berghei and P. vivax liver stages. Data are shown as average ± SD of three to four independent experiments.
(B) Dose-response curve of auphen inhibition of P. vivax schizont growth area.
(C) Dose-response curve of auphen inhibition of P. vivax hypnozoite quantity per well. Data are shown as the average ± SEM of all independent experiments.
(D) Representative confocal images of P. vivax parasites on day 8 post-infection of PHH in the absence (top panel) or presence of 0.62 μM auphen (bottom panel). Arrow indicates parasite with AQP3 recruitment after auphen treatment. Cells were stained with DAPI (blue), PvUIS4 (green), and HsAQP3 (red). Scale bar, 10 μm.