Figure 6.

Phase angle measurements for silicone embedded osmium complex demonstrating the independence of phase angle measurements to room light and other disturbances: During a regular experiment in which a silicone membrane incorporating the osmium dye was being subjected to alternating oxygen and nitrogen sparging, while recording the fluorescence lifetime (phase angles) and fluorescence intensity, a colleague tripped over the tubes supplying the gases (Instant A). After a few moments of attempting to fix the gas supply to the cuvette in the dark, the room lights were turned on (Instant B) and upon correcting the problem, the lights were turned back off again (Instant C).