Table 2.

Intra- and Inter- assay reproducibility of the triplex real-time PCR

Name	Number of DNA Copies (copies/μL)	Intra-assay			Inter-assay
		Mean	SD	CV (%)	Mean	SD	CV (%)
AIV	1 × 107	13.71	0.27	1.97	13.52	0.60	4.44
	1 × 105	20.54	0.22	1.07	20.22	0.25	1.24
	1 × 103	25.97	0.35	1.35	26.14	0.61	2.33
NDV	1 × 107	14.72	0.07	0.48	14.64	0.27	1.84
	1 × 105	21.44	0.31	1.45	21.50	0.34	1.58
	1 × 103	28.11	0.35	1.25	27.90	0.76	2.72
DTMUV	1 × 107	14.25	0.12	0.84	14.38	0.33	2.29
	1 × 105	21.35	0.35	1.64	21.46	0.36	1.68
	1 × 103	28.25	0.62	2.19	28.33	0.89	3.14