Table 3.
The detection of the co-infection models by triplex real-time PCR
| Co-infection proportion a | Number of DNA copies (copies/μL) | Co-infection real-time PCR Ct Value (mean ± SD) b | ||||
|---|---|---|---|---|---|---|
| AIV | NDV | DTMUV | AIV | NDV | DTMUV | |
| AIV:NDV:DTMUV = 10:1:1 | 1 × 107 | 1 × 106 | 1 × 106 | 13.66 ± 0.13 | 18.15 ± 0.27 | 18.15 ± 0.27 |
| AIV:NDV:DTMUV = 10:5:1 | 1 × 107 | 5 × 106 | 1 × 106 | 13.50 ± 0.28 | 17.25 ± 0.27 | 18.03 ± 0.16 |
| AIV:NDV:DTMUV = 1:5:10 | 1 × 106 | 5 × 106 | 1 × 107 | 16.58 ± 0.22 | 17.13 ± 0.15 | 14.54 ± 0.28 |
| AIV:NDV = 1:1 | 1 × 107 | 1 × 107 | – | 13.28 ± 0.48 | 14.43 ± 0.33 | – |
| AIV:NDV = 10:1 | 1 × 107 | 1 × 106 | – | 13.57 ± 0.22 | 18.25 ± 0.23. | – |
| AIV:NDV = 100:1 | 1 × 107 | 1 × 105 | – | 13.93 ± 0.10 | 21.73 ± 0.38 | – |
| AIV:DTMUV =1:1 | 1 × 107 | – | 1 × 107 | 13.43 ± 0.32 | – | 14.38 ± 0.34 |
| AIV:DTMUV =10:1 | 1 × 107 | – | 1 × 106 | 13.57 ± 0.29 | – | 17.92 ± 0.32 |
| AIV:DTMUV =100:1 | 1 × 107 | – | 1 × 105 | 13.24 ± 0.34 | – | 21.33 ± 0.18 |
| NDV:DTMUV =1:1 | – | 1 × 107 | 1 × 107 | – | 14.32 ± 0.32 | 14.53 ± 0.22 |
| NDV:DTMUV =10:1 | – | 1 × 107 | 1 × 106 | – | 14.49 ± 0.19 | 18.02 ± 0.14 |
| NDV:DTMUV =100:1 | – | 1 × 107 | 1 × 105 | – | 14.54 ± 0.15 | 21.68 ± 0.23 |
a There were two kinds of co-infection models included:the triplex co-infection and duplex co-infection
b The data was shown as the mean ± SD (n = 3)
-: represents no design in the corresponding system