Table 4.
Results of the clinical positive samples detected by triplex real-time PCR, conventional PCR and virus isolation
| Positive samples | AIV | NDV | DTMUV | ||||||
|---|---|---|---|---|---|---|---|---|---|
| triplex real-time PCR | Conventional PCR | Virus isolation | triplex real-time PCR | Conventional PCR | Virus isolation | triplex real-time PCR | Conventional PCR | Virus isolation | |
| 1 | + | + | + | – | – | – | – | – | – |
| 2 | + | + | + | – | – | – | – | – | – |
| 3 | + | + | + | – | – | – | – | – | – |
| 4 | + | + | + | – | – | – | – | – | – |
| 5 | + | + | + | – | – | – | – | – | – |
| 6 | + | + | + | – | – | – | – | – | – |
| 7 | + | + | + | – | – | – | – | – | – |
| 8 | + | + | + | – | – | – | – | – | – |
| 9 | + | + | + | – | – | – | – | – | – |
| 10 | + | + | + | – | – | – | – | – | – |
| 11 | + | + | + | – | – | – | – | – | – |
| 12 | + | + | + | + | + | + | – | – | – |
| 13 | + | + | + | + | + | + | – | – | – |
| 14 | + | – | + | – | – | – | – | – | – |
| 15 | + | – | + | – | – | – | – | – | – |
| 16 | + | – | + | – | – | – | – | – | – |
| 17 | + | – | – | – | – | – | – | – | – |
| 18 | – | + | – | – | – | – | – | – | – |
| 19 | – | + | – | – | – | – | – | – | – |
| 20 | – | + | – | – | – | – | – | – | – |
| 21 | – | + | – | – | – | – | – | – | – |
| 22 | – | + | – | – | – | – | – | – | – |
| 23 | – | – | – | + | + | + | – | – | – |
| 24 | – | – | – | + | + | + | – | – | – |
| 25 | – | – | – | + | + | + | – | – | – |
| 26 | – | – | – | + | – | + | – | – | – |
| 27 | – | – | – | + | + | – | – | – | – |
| 28 | – | – | – | – | + | – | – | – | – |
| 29 | – | – | – | – | – | – | + | + | + |
| 30 | – | – | – | – | – | – | + | + | + |
| 31 | – | – | – | – | – | – | + | + | + |
|
Positive rates (%) |
14.17 (17/120) |
15.00 (18/120) |
13.33 (16/120) |
5.83 (7/120) |
5.83 (7/120) |
5.00 (6/120) |
7.50 (3/40) |
7.50 (3/40) |
7.50 (3/40) |