Table 6.
Primers and Probes
| Name a | Sequence (5′-3′) b | Genomic region | Target genes | Amplicon size(bp) |
|---|---|---|---|---|
| AIV-F | AGGGTTTGTGTTCACGCTC | 180–198 | 186 | |
| AIV-R | CCGGTTGAGTAGCTGAGTGC | 346–365 | M | |
| AIV-P | ROX - CCGTGCCCAGTGAGCGAGGAC - BHQ1 | 210–233 | ||
| NDV-F | GACTCAACTCTTGGGCATACA | 837–857 | 172 | |
| NDV-R | TGAGGTGTCAAGCTCTTCTAT | 988–1008 | F | |
| NDV-P | FAM – CAGTCGGGAACCTAAATAATATGCGTGC - BHQ-1 | 872–899 | ||
| DTMUV-F | CAGAGACTGGTTTCATGA | 642–659 | 122 | |
| DTMUV-R | AAGCCACCACTGATTGTT | 746–763 | E | |
| DTMUV-P | JOE - TTACCATGGACAGGGTCATCAGC - BHQ-2 | 667–689 |
a F: forward primer, R: reverse primer, P: TaqMan probe
b ROX: 6-carboxy-x-rhodamine, FAM: 6-carboxy-fluorescein, BHQ-1:Black Hole Quencher 1, BHQ-2: Black Hole Quencher