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. 2020 Jun 19;20:284. doi: 10.1186/s12870-020-02497-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Position of target sites of the sgRNAs in SlPMR4. a. Representation of genomic sequence of SlPMR4 showing the position of four single guide RNAs (sgRNAs) that were designed to produce knock-out mutants of SlPMR4. PCR primers Fw519 + Rv1925 and Fw2969 + Rv4230 were used to identify mutants. b. PROTTER [31] representation of the SlPMR4 protein. sgRNA6 (green) is located in the N-terminal FKS1dom1 domain of the protein; sgRNA8 (yellow), sgRNA1 (orange) and sgRNA7 (red) target sites in the intracellular part of the Glucan-synthase domain