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. 2020 Jun 19;20:284. doi: 10.1186/s12870-020-02497-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Phenotypic response of the pmr4 mutants to infection with Oidium neolycopersici. a. Powdery mildew symptoms observed on the leaves of wild-type plants and mutants (one genotype is given from each of the five mutation events). Photos were taken at 21 days post inoculation (dpi). b. Average disease index score of the mutant lines at 10 and 12 dpi. c. Relative fungal biomass quantification on at least three individual plants of the mutant lines. This is calculated as the ratio of fungal ITS gene amplification in comparison with tomato EF1a and normalized with the values of the wild-type genotype TV161196. Significant differences were observed between the different mutation events and the control lines (Tukey HSD test, α = 0.05)