Table 2.
Genotype | SlPMR4 mutation | Mutation event | Primary AP per IU (%) | Per AP | # Hyphae per IU (%) | ||||
---|---|---|---|---|---|---|---|---|---|
Primary HS (%) | Primary HR (%) | 1 | 2 | 3 | 4 | ||||
MM | wild type | none | 100 | 80 | 22 | 20 | 8 | 30 | 42 |
TV171009 | bi-allelic | 1 + 5 | 88 | 11.3* | 81.8 | 90 | 10 | 0 | 0 |
TV171010 | homozygous | 4 | 96 | 47.9 | 91.6* | 56 | 16 | 26 | 2 |
TV161212 | bi-allelic | 2 + 3 | 92 | 36.9 | 84.7* | 68 | 16 | 12 | 4 |
Development of Oidium neolycopersici on the susceptible genotype Moneymaker (MM) and three slpmr4 CRISPR mutant lines carrying different mutation events
Asterisks represent statistically significant differences between the mutant genotypes and cultivar MM as calculated by a t-test. *: p < 0.05
IU Infection unit, AP Appressorium, HS Haustorium, HR Hypersensitive response-like cell death