Fig. 3.

Paramylon induces TNF-α and IL-6 secretion. RAW264.7 cells were incubated with the different concentrations of paramylon shown for 12 h (for RNA test) or 24 h (for protein test). The production of TNF-α (a) and IL-6 (b) in the culture supernatant was detected by ELISA. The mRNA levels of the same genes were also analyzed by qRT-PCR (c, d). Representative results from three independent experiments are shown. * P < 0.05, ** P < 0.01, *** P < 0.001