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. 2020 Jun 19;11:52. doi: 10.1186/s13229-020-00351-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Neurons lacking FMRP have reduced I_BKCa densities and their burst properties are insensitive to paxilline. a Large Ca-activated potassium currents (I_BKCa) evoked by a depolarizing voltage ramp from −110 to +40 mV, 10 mV/s, with a hold of 50 ms at +40 mV. b The I_BKCa was isolated by subtracting the currents recorded in the absence and presence of paxilline (10 μM). c I–V curves plotted from the ramp-evoked I_BKCa showing the decreased I_BKCa in neurons lacking FMRP. Currents are normalized to the corresponding cell capacitance. d Quantification of current densities at + 40 mV show significant decrease of I_BKCa in neurons lacking FMRP. Overall, the mean current densities are 11.73 ± 1.49 (FMR1^+/y, n = 15, N = 3); 7.4 ± 1.43 (CON2, n = 14, N = 3); 3.57 ± 0.62 (FMR1^−/y, n = 14, N = 3); 1.44 ± 0.78 (FXS3, n = 12, N = 3). *p < 0.05, ***p < 0.001, two-way repeated measures ANOVA with post hoc Tukey’s test, paired t test, Wilcoxon test. e Representative traces illustrating spontaneous bursts recorded from either a FMR1^+/y neuron or a FMR1^−/y neuron before and after the application of paxilline (10 μM) and illustrating the increase of action potential burst frequencies in FMR1^+/y neurons in the presence of paxilline. f Quantification of the number of bursts recorded from FMR1^+/y, CON2, FMR1^−/y, or FXS3 neurons before (open symbols) or after (closed symbols) application of paxilline. Overall, the mean number of bursts are 6.7 ± 1.086 (FMR1^+/y, n = 10, N = 3); 13.8 ± 1.85 (FMR1^+/y + paxilline, n = 10, N = 3); 5.6 ± 1.08 (CON2, n = 10, N = 3); 11.3 ± 1.94 (CON2 + paxilline, n = 10, N = 3); 25.5 ± 4.51 (FMR1^−/y , n = 10, N = 3); 26 ± 5.12 (FMR1^−/y + paxilline, n = 10, N = 3); 25.4 ± 6.6 (FXS3, n = 10, N = 3); 26.5 ± 5.95 (FXS3 + paxilline, n = 10, N = 3). g Quantification of the duration of bursts recorded from FMR1^+/y, CON2, FMR1^−/y, or FXS3 neurons before (open symbols) or after (closed symbols) application of paxilline. Overall, mean burst duration are 40.97 ± 8.68 s (FMR1^+/y); 38.47 ± 7.85 s (FMR1^+/y + paxilline); 58.65 ± 13.12 s (CON2); 56.08 ± 14.04 s (CON2 + paxilline); 2.65 ± 0.53 s (FMR1^−/y); 3.08 ± 0.68 s (FMR1^−/y + paxilline); 4.107 ± 0.64 s (FXS3); 3.93 ± 0.77 (FXS3 + paxilline). **p < 0.01, ***p < 0.001, paired t test, Wilcoxon test