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. Author manuscript; available in PMC: 2020 Jun 19.
Published in final edited form as: Cell Rep. 2020 May 19;31(7):107662. doi: 10.1016/j.celrep.2020.107662

Figure 1. Forward Genetic Screen to Isolate Mutants Susceptible to Infection-Associated Dendrite Degeneration.

Figure 1.

(A) Schematic diagram of the forward genetic screen. Animals (P0) were treated with ethylmethanesulfonate (EMS) and the F1 animals were self-fertilized. The F2 animals were analyzed for dendrite degeneration, and animals exhibiting any changes were discarded. The remaining mutants were infected with P. aeruginosa for 24 h and screened for dendrite degeneration.

(B) Representative photomicrographs of dendrite degeneration in control animals expressing RFP under the control of odr-1 promoter (CX5974) (top panel) and two representative mutants (#23 and #15) (bottom panels) after exposed to P. aeruginosa for 24 h. The scale bars represent 20 µm. White asterisk indicates bead-like structures and wavy dendrites.

(C) Quantification of dendrite degeneration in control and different mutant animals after 24 h of infection.