| Bacterial and Virus Strains |
|
|
Escherichia coli OP50 |
Caenorhabditis Genetics Center |
Wormbase ID: OP50 |
|
Pseudomonas aeruginosa PA14 |
Laboratory of Frederick M. Ausubel |
PA14 |
| Ahringer RNAi Libraries in E. coli HT115 (DE3) |
Source BioScience |
N/A |
|
Enterococcus faecalis OGIRF |
Laboratory of Danielle A Garsin |
OGIRF |
|
| Chemicals, Peptides, and Recombinant Proteins |
|
| Ethyl-methanesulfonate (EMS) |
Millipore Sigma |
Catalog No. M0880 |
| Isopropyl-β-D-thiogalactoside |
Anatrace |
Catalog No. I1003 |
| Proteinase K |
QIAGEN |
Catalog No. 19131 |
| KpnI Restriction enzyme |
NEW ENGLAND Biolabs |
Catalog No. R0142S |
| SalI-HF Restriction enzyme |
NEW ENGLAND Biolabs |
Catalog No. R3138S |
|
SphI-HF Restriction enzyme |
NEW ENGLAND Biolabs |
Catalog No. R3182S |
| BamHI Restriction enzyme |
NEW ENGLAND Biolabs |
Catalog No. R3136S |
| T4 DNA Ligase |
NEW ENGLAND Biolabs |
Catalog No. M0202S |
| Phusion High-Fidelity PCR Kit |
NEW ENGLAND Biolabs |
Catalog No. M0531S |
| Dream Taq Green PCR Master Mix (2X) |
Thermo Fisher Scientific |
Catalog No. K1081 |
|
| Critical Commercial Assays |
|
| Gentra Puregene kit |
QIAGEN |
Catalog No. 158667 |
| RNeasy Plus Universal Kit |
QIAGEN |
Catalog No. 73404 |
| TURBO DNase |
Life Technologies |
Catalog No. AM1907 |
| High-Capacity cDNA Reverse Transcription Kit |
Applied Biosystems |
Catalog No. 4368814 |
| Power SYBR PCR Master Mix |
Applied Biosystems |
Catalog No. 4367659 |
|
| Deposited Data |
|
| Raw and analyzed mRNA-Seq data |
This study |
GEO: GSE137112
|
|
| Experimental Models: Organisms/Strains |
|
| N2 C.elegans wild isolate |
Caenorhabditis Genetics Center |
WormBase ID: N2 |
|
unc-86::myr GFP + odr-1::RFP |
Caenorhabditis Genetics Center |
Strain CX5974 |
| F49H12.4::GFP + unc-119(+) |
Caenorhabditis Genetics Center |
Strain NC1686 |
| fer-1(b232ts) |
Caenorhabditis Genetics Center |
Strain HH142 |
| kyIs104 |
Caenorhabditis Genetics Center |
Strain CX3553 |
| daf-16 (mu86) |
Caenorhabditis Genetics Center |
Strain CF1038 |
| daf-16p::daf-16a/b::GFP + rol-6(su1006) |
Caenorhabditis Genetics Center |
Strain TJ356 |
| unc-86::myr GFP + odr-1::RFP;F49H12.4::GFP + unc-119(+) |
This study |
N/A |
| srbc-48(ac23);F49H12.4::GFP + unc-119(+) |
This study |
N/A |
|
unc-86::myr GFP + odr-1::RFP; daf-16p::daf-16a/b::GFP + rol-6(su1006) |
This study |
N/A |
|
srbc-48(ac23); daf-16p::daf-16a/b::GFP + rol-6(su1006) |
This study |
N/A |
|
odr-1p::srbc-48::GFP;daf-16p::daf-16a/b::GFP + rol-6(su1006) |
This study |
N/A |
| srbc-48p::srbc-48;daf-16p::daf-16a/b::GFP + rol-6(su1006) |
This study |
N/A |
| srbc-48(ac23);daf-16 (mu86) |
This study |
N/A |
|
| Oligonucleotides |
|
| See Table S6 for the primers used in the study |
Integrated DNA Technologies |
N/A |
|
| Recombinant DNA |
|
| srbc-48p::srbc-48 |
This study |
N/A |
|
odr-1p::srbc-48::GFP |
This study |
N/A |
|
odr-1p::RFP |
This study |
N/A |
|
srbc-48p::GFP |
This study |
N/A |
|
odr-1p::RFP; Psrbc-48::GFP |
This study |
N/A |
|
| Software and Algorithms |
|
| Prism7 |
Graph Pad Software, La Jolla, CA |
https://www.graphpad.com/scientificsoftware/prism/ |
| Snapgene |
GSL Biotech |
https://www.snapgene.com |
| ImageJ |
NIH |
https://imagej.nih.gov/ij/ |
| Galaxy |
N/A |
https://usegalaxy.org |
| Database for Annotation, Visualization, and Integrated Discovery (DAVID) |
N/A |
https://david.ncifcrf.gov/ |
