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. 2020 Jun 12;11:1301. doi: 10.3389/fmicb.2020.01301

FIGURE 5.

FIGURE 5

S. aureus infection activates the expression of TWIST1 in Raw 264.7 cells. (A) qPCR analysis of the mRNA expression of TWIAT1, NANOG, and ERBB2 in Raw 264.7 cells in response to S. aureus infection with MOI [the ratio of the S. aureus colonies number (CFU) to the Raw 264.7 cells number] at 0.01, 0.1, 1.0, and 10. ANOVA followed by Dunnett’s test, n = 3/group. (B) Immunoblot and (C) quantitative analysis of TWIST1 in Raw 264.7 cells after infection. Two-tailed Student’s t test, n = 3/group. (D) qPCR analysis of the effect of siRNA fragments on the mRNA expression of TWIST1 in Raw 264.7 cells. ANOVA followed by Dunnett’s test, n = 3/group. (E) Immunoblot and (F) quantitative analysis of the effect of siRNA fragments on the protein expression of TWIST1 in Raw 264.7 cells. ANOVA followed by Dunnett’s test, n = 3/group. (G) qPCR analysis of the effect of knocking down TWIST1 on the mRNA expression of marker genes for macrophage polarization in Raw 264.7 cells after S. aureus infection. ANOVA followed by Dunnett’s test, n = 3/group. *p < 0.05, **p < 0.01, and ***p < 0.001.