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. 2020 Jun 12;8:190. doi: 10.3389/fpubh.2020.00190

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Copyright © 2020 Norton, Williams, Falkinham and Honda.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Repeated UV exposure does not affect the susceptibility of NTM to UV-C. The sensitivity to UV-C treatment between unexposed (water acclimated, top row) M. abscessus, M. avium, and M. chimaera and 56-day UV-adapted M. abscessus, M. avium, and M. chimaera (56-days UV-adapted, bottom row) were compared for their ability to survive 90 s of UV-C irradiation. One hundred microliter of bacterial stock was spread onto 7H10 agar plates. Half of the plate was covered with by notecard to protect against UV-exposure and the other half was exposed directly to 254-nm UV-C light for 90 s. Plates were incubated at 37°C and the growth of the UV-C treated (above red line) and untreated (below red line) regions were visually compared.