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. 2020 Jun 12;11:814. doi: 10.3389/fpls.2020.00814

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Copyright © 2020 Wang, Chen, Yang, Sun, Ding, Li, Xu and Tan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PPR14 is a P-type PPR protein localized in mitochondria. (A) The predicted PPR14 protein contains 14 PPR motifs. (B) Predicted structure of PPR14 protein. This model was predicted using Phyre2 (http://www.sbg.bio.ic.ac.uk/phyre2/html/page.cgi?id=index) based on template PPR10. Blue alpha helices indicate the N-terminus and red helices indicate the C-terminus of PPR14. (C) Phylogenetic analysis of PPR14 proteins in different species. (D) Subcellular localization of PPR14 protein. A PPR14^N301-GFP fusion protein was transiently expressed in epidermal cells of N. benthamiana leaves. Mitochondria were stained by MitoTracker. Fluorescence signals from PPR14^N301-GFP, MitoTracker, and chloroplast autofluorescence were detected by a confocal laser microscope. DIC, differential interference contrast; N301, the N terminus 301 amino acids of PPR14. Bars = 20 μm.