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. 2020 Jun 12;11:814. doi: 10.3389/fpls.2020.00814

FIGURE 2.

FIGURE 2

Phenotype of the ppr14 mutant. (A) Gene structure of PPR14 and locations of the Mu insertions in two independent alleles. The black box represents protein translated region, and white boxes represent the 5′ and 3′ untranslated regions. Mu insertion sites of ppr14 alleles are indicated by triangles. (B) The selfed ear segregates 3:1 for wild-type and ppr14-1 mutants (green arrows). (C) Comparison of mature wild-type (WT) and ppr14-1 mutant kernel. (D,E) Dissection of WT and ppr14 kernels at 13 (D) and 21 (E) days after pollination (DAP). en, endosperm; em, embryo. (F) RT-PCR analysis of PPR14 expression in ppr14 mutant alleles and WT seeds at 17 DAP. Bars = 1 cm in (B), 5 mm in (C), 1 mm in (D,E).