FIGURE 5.

PPR14 is involved in the splicing of nad2 intron 3 and nad7 introns 1 and 2. (A) Detailed RT-PCR analysis of nad2 and nad7 splicing. ZmActin was used to normalize the quantifications. S, intron spliced; U, intron unspliced. (B) QRT-PCR analysis of 22 introns of mitochondrial gene transcripts in the ppr14-1 mutant and wild-type (WT). RNA was extracted from ppr14-1 mutant and WT kernels of three independent ears. ZmEF1α was used to normalize the quantifications. Values and error bars represent the mean and standard deviation of three biological replicates, respectively.