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. Author manuscript; available in PMC: 2021 May 15.
Published in final edited form as: J Immunol. 2020 Apr 3;204(10):2829–2839. doi: 10.4049/jimmunol.1901227

Figure 5, SP-A inhibits IL-13-induced STAT3 phosphorylation in asthma.

Figure 5,

Human bronchial epithelial cells (BECs) obtained from asthmatic and normal participants were grown at an ALI for 2 weeks prior to IL-13 challenge (10 ng/ml) in the presence or absence of control oligomeric SP-A (20 μg/ml). A) Total cell lysates were analyzed for STAT3 or STAT6 phosphorylation determined by Western blot and compared to total STAT3 or STAT6 30 min after IL-13 challenge. Western blots are representative of n=3 asthma and 3 normal participants on two independent blots. B) Muc5AC gene expression was determined after stimulation with IL-13 using a 5 day protocol (10 ng/ml at 48 hours x 2, with trypsinization at 5 days) and C) IL-8 secretion were examined 48 hours from BEC that were challenged with IL-13 in the presence or absence of either SP-A or a STAT3 inhibitor added 30 minutes prior to IL-13.