Table 1. Primers used in this study.

Primer	Genes	Sequence (5′ → 3′)	Size of PCR-product (bp)	Primer concentration (pmol/ul)	Reference
HRM MultiplexfljB, gyrB and ycfQ genes (HRM-rt PCR)
fljB _f	fljB	GTGAAAGATACAGCAGTAACAACG	170	0.075	Zeinzinger et al. (2012)
fljB _r		CAAAGTACTTGTTATTATCTGCG		0.075	Zeinzinger et al. (2012)
gyrB _f	gyrB	AAACGCCGATCCACCCGA	171	0.1	Zeinzinger et al. (2012)
gyrB _r		TCATCGCCGCACGGAAG		0.1	Zeinzinger et al. (2012)
ycfQ _f	ycfQ	GCCTACTCTCTATGCGGAATTCAC	241	0.075	Zeinzinger et al. (2012)
ycfQ _r		GATATCGCGCGAGGAGGCG		0.075	Zeinzinger et al. (2012)
CRISPR 2 Monoplex
B1	CRISPR 2 loci	GAGCAATACYYTRATCGTTAACGCC	Variable	0.2	Fabre et al. (2012)
B2		GTTGCDATAKGTYGRTRGRATGTRG		0.2	Fabre et al. (2012)
Multiplex 1blaTEM variants including TEM-1 and TEM-2, blaSHV variants including SHV-1, blaOXA-1-like including OXA-1, OXA-4 and OXA-30
TEM_f	blaTEM	CATTTCCGTGTCGCCCTTATTC	800	0.4	Dallenne et al. (2010)
TEM_r		CGTTCATCCATAGTTGCCTGAC		0.4	Dallenne et al. (2010)
SHV_f	blaSHV	AGCCGCTTGAGCAAATTAAAC	713	0.4	Dallenne et al. (2010)
SHV_r		ATCCCGCAGATAAATCACCAC		0.4	Dallenne et al. (2010)
OXA_f	blaOXA	GGCACCAGATTCAACTTTCAAG	564	0.4	Dallenne et al. (2010)
OXA_r		GACCCCAAGTTTCCTGTAAGTG		0.4	Dallenne et al. (2010)
Multiplex 2blaCTX-M group 1 and group 9; variants of blaCTX-M group 1 including CTX-M-1, CTX-M-3 and CTX-M-15, variants of blaCTX-M group 9 including CTX-M-9 and CTX-M-14
CTX 1_f	blaCTX-M group 1	TTAGGAARTGTGCCGCTGYAa	688	0.4	Dallenne et al. (2010)
CTX 1_r		CGATATCGTTGGTGGTRCCATa		0.2	Dallenne et al. (2010)
CTX 9_f	blaCTX-M group 9	TCAAGCCTGCCGATCTGGT	561	0.4	Dallenne et al. (2010)
CTX 9_r		TGATTCTCGCCGCTGAAG		0.4	Dallenne et al. (2010)
Molecular typing
ERIC_f	ERIC-PCR	ATGTAAGCTCCTGGGGATTCAC	Variable	20	Versalovic, Koeuth & Lupski (1991)
ERIC_r		AAGTAAGTGACTGGGGTGAGCG		20	Versalovic, Koeuth & Lupski (1991)

Notes.

^aY = T or C; R = A or G; S = G or C; D = A or G or T.