Fig. 7.

Transcription of symbiotic GmLbs and non-symbiotic GmHbs in nodules. (A) Relative expression of GmLbs/GmHbs in soybean nodules under LN conditions. (B) Expression of nodule-specific GmLb genes in nodules at 7, 14, 20, 30, 40 and 50 dai. Relative expression values were calculated as the ratio of GmLb expression to that of the soybean housekeeping gene TefS1 (accession number X56856). Relative expression was normalized against the mean of each GmLb transcription level at 7 dai. (C) Expression of nodule-specific GmLb genes in response to excess N supply, shown as the binary logarithm of fold changes in relative expression of GmLb members under HN (20 mm N added) compared with LN (530 μm N added) conditions. Values are mean ± s.e. (n = 4).