Table 4. Comparison of the pre-S genotyping results by IHC- and NGS-based analyses in 30 HBV-related HCC patients.
Patient groups by pre-S genotyping | McNemar’s paired proportion test | Simple Kappa Coefficient | ||||
---|---|---|---|---|---|---|
NGS (−) | NGS (+) | P value | κ value | 95% CI | ||
Patients without (−)/with (+) any type of GGH/pre-S del (n) (%) | IHC (−) | 5 (17)a | 6 (20)b | 0.0143 | 0.51 | 0.20–0.81 |
IHC (+) | 0 (0)c | 19 (63)d | ||||
Patients without (−)/with (+) type I GGH (score 1–4) and type II GGH (score 0–4)/deletion spanning pre-S1 gene segment (n) (%) | IHC (−) | 12 (40) | 5 (17) | 0.0253 | 0.67 | 0.42–0.92 |
IHC (+) | 0 (0) | 13 (43) | ||||
Patients without (−)/with (+) type II GGH (score 1–4) and type I GGH (score 0–4)/deletion spanning pre-S2 gene segment (n) (%) | IHC (−) | 9 (30) | 6 (20) | 0.0143 | 0.60 | 0.33–0.86 |
IHC (+) | 0 (0) | 15 (50) |
aThe number of patients without the indicated GGH and pre-S deletion by both IHC- and NGS-based analyses.
bThe number of patients with the indicated pre-S deletion by NGS- but not IHC-based analysis.
cThe number of patients with the indicated pre-S deletion by IHC- but not NGS-based analysis.
dThe number of patients with the indicated pre-S deletion by both IHC- and NGS-based analyses.
Abbreviations: NGS, next-generation sequencing; GGH, ground glass hepatocytes; IHC, immunohistochemistry; del, deletion; n, number.