Table 5. The pre-S genotyping results by additional IHC-based analysis in selected HBV-related HCC patients.
| Patient No. | IHC Result (GGH Type (Score))a,b | NGS Result (Pre-S Deletion Type (%))d |
|---|---|---|
| 4 | 1. type I GGH (0), type II GGH (0) | 1. pre-S1 del (86.404)e |
| 2. type I GGH (1), type II GGH (0)c | 2. wild-type (12.695) | |
| 3. pre-S2 del (0.737) | ||
| 4. pre-S1+pre-S2 del (0.163) | ||
| 7 | 1. type I GGH (3), type II GGH (0) | 1. pre-S1+pre-S2 del (46.237) |
| 2. type I GGH (3), type II GGH (3) | 2. pre-S2 del (26.927) | |
| 3. pre-S1 del (14.368) | ||
| 4. wild-type (12.467) | ||
| 9 | 1. type I GGH (0), type II GGH (0) | 1. pre-S2 del (56.155) |
| 2. type I GGH (0), type II GGH (0) | 2. wild-type (42.610) | |
| 3. pre-S1+pre-S2 del (0.718) | ||
| 4. pre-S1 del (0.516) | ||
| 13 | 1. type I GGH (0), type II GGH (0) | 1. wild-type (94.701) |
| 2. type I GGH (1), type II GGH (0) | 2. pre-S1 del (5.086) | |
| 3. pre-S2 del (0.137) | ||
| 4. pre-S1+pre-S2 del (0.077) | ||
| 14 | 1. type I GGH (1), type II GGH (0) | 1. wild-type (69.001) |
| 2. type I GGH (1), type II GGH (3) | 2. pre-S1 del (20.530) | |
| 3. pre-S2 del (9.463) | ||
| 4. pre-S1+pre-S2 del (1.006) | ||
| 18 | 1. type I GGH (0), type II GGH (0) | 1. pre-S2 del (41.477) |
| 2. type I GGH (1), type II GGH (4) | 2. pre-S1+pre-S2 del (39.126) | |
| 3. wild-type (12.348) | ||
| 4. pre-S1 del (7.048) | ||
| 20 | 1. type I GGH (0), type II GGH (0) | 1. wild-type (30.944) |
| 2. type I GGH (1), type II GGH (1) | 2. pre-S2 del (30.409) | |
| 3. pre-S1+pre-S2 del (29.105) | ||
| 4. pre-S1 del (9.542) | ||
| 26 | 1. type I GGH (0), type II GGH (0) | 1. wild-type (30.973) |
| 2. type I GGH (0), type II GGH (0) | 2. pre-S1+pre-S2 del (27.774) | |
| 3. pre-S1 del (27.161) | ||
| 4. pre-S2 del (14.091) |
aThe percentage of each type of GGH was scored from 0 to 4 corresponding to 0%, <5%, 5% to 9%, 10% to 29%, and ≥30% of hepatocytes in liver tissues, respectively.
bThe results of two liver tissue specimens (the original one and another one) from each patient were shown in descending order.
cThe result of IHC in correspondence with that of NGS was shown in bold.
dThe total percentage of pre-S gene DNA in each type of pre-S deletion was shown in descending order.
eThe pre-S deletion type above the cut-off percentage (5.049) was shown in bold.
Abbreviations: IHC, immunohistochemistry; NGS, next-generation sequencing; GGH, ground glass hepatocytes; del, deletion.