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LESA single-pass cIM-MS of mouse brain tissue, with the quadrupole set to transmit m/z > 600. (A) Total LESA mass spectrum (summed across the entire ATD). (B) 2D heat-map (arrival time vs m/z). Regions of interest (ROI) containing peaks corresponding to proteins are highlighted. (C) Mass spectra extracted from ROI 1, ROI 2, ROI 3, and ROI 4. Abundant proteins are indicated.
