Table 1.
Group Names | Microinjection into the Lateral Hypothalamus (0.5 μL/rat) | Microinjection into the Ventral Tegmental Area (0.3 μL/rat) | Surgery | |
---|---|---|---|---|
Intact | Subcutaneous injection of 50 μL formalin 1% into the upper lip | - | - | - |
Sham operated | - | - | Surgery and recovery periods (5 to 7 days) | |
Saline-control (SAL/Control) |
Saline | - | ||
Carbachol (CAR) | Carbachol (250 nM) | - | ||
Vehicles (VEHs) |
Saline | Saline or DMSO 12% | ||
Carbachol-control groups (CAR/Control) |
Carbachol (250 nM) | Saline or DMSO 12% | ||
SCH23390-treated groups (SCH/CAR) and (SCH/Saline) |
Carbachol (250 nM) | SCH23390 (0.5 µg) | ||
Carbachol (250 nM) | SCH23390 (1 µg) | |||
Carbachol (250 nM) | SCH23390 (4 µg) | |||
Saline | SCH23390 (4 µg) | |||
Sulpiride-treated groups (SUL/CAR) and (SUL/DMSO) |
Carbachol (250 nM) | Sulpiride (0.5 µg) | ||
Carbachol (250 nM) | Sulpiride (1 µg) | |||
Carbachol (250 nM) | Sulpiride (4 µg) | |||
DMSO 12% | Sulpiride (4 µg) |