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. 2020 Apr 7;21(6):2246–2257. doi: 10.1021/acs.biomac.0c00205

Figure 5.

Figure 5

(A) Synthetic scheme for the substitution of Pfp esters in the polymeric precursor micelle cores with Oregon Green cadaverine and different cross-linkers and/or mPEG-amine as hydrophilizing agent. (B) DLS characterization of poly(Pfp-Nb)50-b-poly(mPEG-Nb)50-derived cross-linked nanogels and non-cross-linked polymers with and without fluorescent dye. (C) UV absorbance of dye-conjugated nanogels and polymers compared to nonlabeled species. (D) Cell viability assay (MTT) of RAW-Blue macrophages incubated with cross-linked nanogels and non-cross-linked polymers at increasing concentrations for 18 h (n = 4). (E) Flow cytometry histograms (1) and mean fluorescence intensities (MFI) (2) of RAW-Blue macrophages incubated with Oregon Green labeled cross-linked nanogels and non-cross-linked polymers at 30 mg/L and 100 μg/mL for 18 h (n = 3). (F) Confocal laser scanning fluorescence microscopy images of RAW-Blue macrophages incubated with Oregon Green labeled cross-linked nanogels and non-cross-linked polymers at 100 μg/mL for 24 h (green: Oregon Green labeled polymer or nanogel species; blue: nuclei stained with DAPI; gray: dark field image for cell morphology; scale bar: 10 μm).