Figure 2.

CD8⁺ dendritic cells (DCs) are decreased in the tumor microenvironments (TMEs) of tumor-bearing Hdac9^-/- mice. The percentages of CD8⁺ or PD-L1⁺ cells in DCs (CD11c⁺ cells) in the TME (A) and draining lymph nodes (DLNs) (B) of Lewis lung carcinoma (LLC) tumor-bearing wild-type (WT) or Hdac9^-/- mice were determined by flow cytometry. (C) The percentages of CD8⁺ T cells in the T cell population (CD3⁺) in the TMEs and DLNs of LLC-bearing mice were determined by flow cytometry. Dot plots were gated on CD45⁺ cells. (D) The mRNA expression levels of antitumor immune response-related molecules in the TME were determined by quantitative reverse transcription-PCR and calculated by the 2^-ΔCt method. Representative results from three independent experiments are shown. Data represent the mean±SEM. Statistical significance was determined by two-sided unpaired Student’s t-test. *p<0.05. n.s. means no significant difference.