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. 2020 Jun 17;8(1):e000612. doi: 10.1136/jitc-2020-000612

Figure 3.

Figure 3

Correlation between tumor weight and immune cell infiltration per milligram of tumor in the TC-1 syngeneic model. TC-1 tumor bearing female C57BL/6J mice were treated with PBS control (100 µL s.c.), PDS0101 (s.c., 3 weekly doses starting on day 7), bintrafusp alfa (250 µg, i.p., days 7, 9 and 11), and/or NHS-IL12 (50 µg, s.c., day 7). (A) A smaller tumor volume correlated with increased CD4+ and CD8+ T cells, regulatory T cells (Treg), and M1 macrophage infiltration into the tumor. Data are shown for all treatment groups combined. (B) Immunohistochemistry for CD4+ and CD8+ T cells in TC-1 tumors. Tumors were harvested, blocked, sectioned and stained with the Opal Immunology kit. Combination treatment with PDS0101, NHS-IL12, and bintrafusp alfa increased CD8+ and CD4+ T cell infiltration into the tumor compared with monotherapy treatments. IL12, interleukin-12; i.p. intraperitoneally; s.c., subcutaneously.