Fig. 2.

Ficoll-purified PBMC were stained for CD3 and CD8, then gated on (a) CD3⁺ or (d) CD3⁻ cells and then analysed for percentage of CD3⁺ and CD3⁻ cells along with (b) CD3⁺CD4⁺, (c) CD3⁺CD8⁺, (e) CD3⁻CD8⁻ and (f) CD3⁻CD8⁺ lymphocytes in gate 1. CD3⁺CD4⁺ cells were represented by the CD3⁺CD8⁻ subset in the CD3⁺ lymphocyte gate. Statistical analysis was carried out using the non-parametric Wilcoxon rank sum test. Circles and asterisks are outlier values. All asterisks represent outlier values, which lay outside the box at a distance of 1.5x the box size. All circles represent outlier values greater than the distance of 3x the box size.