Fig. 3.

PBMC were stained for CD3, CD8 and one of the following: Foxp3, IL17 and IFN-γ. Box and whisker plots showing the range of CD3⁺CD4⁺ PBMC which co-express (a) Foxp3 and (b) IL17 in each group. Circles and asterisks are outlier values. All asterisks represent outlier values, which lay outside the box at a distance of 1.5x the box size. All circles represent outlier values greater than the distance of 3x the box size. (c). The percentage of CD3⁺CD4⁺ cells which co-expressed IL17 and Foxp3 or IFN-γ was plotted in order to examine the pro-inflammatory or regulatory nature of the CD3⁺CD4⁺ population for each individual. Circles represent the six patients whose blood was taken at the time of primary CDI, but who later became recurrent. Striped circles or diamonds represent plasticity of co-expression, and white diamonds or circles represent skewed IL17 co-expression.