Skip to main content
. 2020 Apr 8;14(5):5711–5727. doi: 10.1021/acsnano.0c00326

Figure 1.

Figure 1

Synthesis of high MW pABOL and characterization of resulting saRNA polyplexes. (a) Polymerization kinetics of ABOL with CBA under different reaction conditions with varying monomer concentration, with and without triethylamine (TEA). The conversion values were calculated from the NMR integrals of double-bond signals at 5.60–6.23 ppm, using the methylene signals at 1.36–1.47 ppm as the internal reference. These methylene signals are assigned to g and h in Figure S2, which remain constant during the polyaddition (see Figure S4 for details). (b and c) Particle diameter and zeta potential of polyplexes formed via the direct mixing method between pABOLs and saRNA at polymer to saRNA weight ratios ranging from 1 to 45. PEI-44 [poly(ethylene imine), linear, 44 kDa] at a weight ratio of 5:1 was used as the reference. Bar represents mean ± SD for n = 3 with the weight ratio of polymer:saRNA indicated above the bars. (d) Typical TEM of polyplexes (pABOL-100/saRNA = 45:1, w/w) stained with 2 wt % uranyl acetate (scale bar: 100 nm; more images in Figure S6).