Figure 7.

Immunogenicity of HA-encoding saRNA polyplexes. (a, b) Change in body weight after IN challenge with Cal/09 flu virus for mice injected either IM (a) or ID (b). Dots represent mean percentage, normalized to day 0 for each mouse, ±SEM for n = 5. § indicates significance of p < 0.05 for 1 μg dose vs naïve, while # indicates significance of p < 0.05 for 1 μg dose PEI group vs 1 μg dose pABOL group as evaluated using multiple t tests adjusted for multiple comparisons. (c, d) HA antigen-specific IgG antibody titers following immunization with prime and boost of saRNA complexed with jetPEI, 8 kDa pABOL, or 100 kDa pABOL for mice injected either IM (c) or ID (d). Data are presented as box and whiskers with outer limits of the minimum and maximum, and a line as the mean for n = 5. (e, f) HAI titer of Cal/09 flu virus for mice injected either IM (e) or ID (f). Gray dotted line represents the limit of detection. (g, h) Neutralization IC₅₀ against Cal/09 flu virus for mice injected either IM (g) or ID (h). * indicates significance of p < 0.05 as evaluated using a Kruskal–Wallis test with multiple comparisons. Each bar represents mean ± SEM for n = 5 at each time point.