Extended Data Fig. 4. CD8⁺ T cell dependent Katushka signaling in IGS reporter cells in vivo.

a) Flow cytometric analysis of Katushka expression in CFP⁺ IGS reporter (left panel) and GFP⁺ Ag⁺ (right panel) cells derived from mixed tumors described in Fig. 2b. Data from mice treated with HBSS are depicted in blue, data from mice treated with CDK4_R>L-specific CD8⁺ T cells are depicted in red, n=4 mice per condition, data obtained from one experiment. b) Representative images of tumors before and 120h after injection of CDK4_R>L-specific CD8⁺ T cells (left panel, three independent experiments with n=1 mouse each) or HBSS (right panel, two independent experiments with n=1 mouse each), for the imaging experiments described in Fig. 3. SHG: Second-harmonic generation. Scale bar is 200 mm.