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. 2020 Jun 19;11:3138. doi: 10.1038/s41467-020-16900-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Expression data of all extracts used in mass spectrometry analysis. Data are presented as mean values and error bars represent s.d. (n = 6 three independent reactions of two independent extract preparations). Standard two-tail t-test. b Principal component analysis showing the grouping of the various extracts based on their protein profile. Ellipses indicate the boundary for statistical significance by a two-tailed t-test (p value < 0.01). Clear grouping is observed between the replicates of each sample. c Volcano plots displaying the changes in protein intensity between each extract and the BL21(DE3) control. Red data points indicate a decrease in protein intensity greater than 25% and a p value < 0.01 from a two-sided t-test. Blue data points indicate an increase in protein intensity greater than 25% and a p value < 0.01 form a two-tailed t-test. The proteins that were intentionally overexpressed are colored black. Numerous statistically significant changes are observed, many of which are downregulated. d The sum of protein intensity in each category for each extract is presented as percentages of the total protein intensity in that extract. There is a marked increase in gene expression and a decrease in metabolism and homeostasis related proteins when comparing BL-1S and BL-7S to BL-E. e The identified proteins were further subdivided into more specific functional groups. The log difference between the protein intensity of each extract and BL-E were calculated and then averaged within each functional group and plotted on a colorimetric scale. The dendrogram represents the clustering of each protein group based on their similarity to other groups. CFP was inserted into the Unknown category. f Diagram of translation indicating the key changes in the proteome and their influence on gene expression. Source data for a–e are provided as a Source Data file. In b–e use the same samples throughout. (n = 4 two independent samples from two independent extract preparations).