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. 2020 Mar 23;14(7):1627–1638. doi: 10.1038/s41396-020-0633-3

Fig. 2. Closing of the constricted region.

Fig. 2

a At 24 h after inoculation, the CR and the M4B region are completely closed. b LSM observations reveal that epithelial cells of M4B are bulged at the luminal side and closed like a zipper during symbiont colonization. c At 72 h after inoculation, symbiont cells flow back into M4B, but never flow over into M3. d LSM observations indicate that the CR (arrowheads) is kept closed after the reopening of M4B. In a and c, host nuclei are stained by DAPI, shown in blue. In b and d, host nuclei and cytoskeleton are stained with SYTOX green (green) and phalloidin (red), respectively.