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. 2020 Mar 23;14(7):1627–1638. doi: 10.1038/s41396-020-0633-3

Fig. 3. The closure of the constricted region and M4B during symbiont colonization in 3rd instar nymphs of R. pedestris.

Fig. 3

a Infection scheme. b–g No morphological alteration in the M4B of aposymbiotic 2nd instar insects. Asterisks indicate the lumen of M4B. h–m A GFP-labeled Burkholderia symbiont was orally administered to aposymbiotic 3rd instar nymphs and their M4B morphogenesis was investigated. Merged fluorescence and DIC images are shown. Abbreviations are as in Fig. 1.