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. 2020 Mar 23;14(7):1627–1638. doi: 10.1038/s41396-020-0633-3

Fig. 5. The midgut closure is irreversible.

Fig. 5

a Infection schemes for three series of experiments. In the Cm-RFP control experiment, 2nd instar insects are kept aposymbiotic and fed with chloramphenicol (Cm) in the drinking water; after molting, these insects are infected with the RFP strain. In the GFP-RFP control experiment, 2nd instar insects are infected with the GFP strain present in the drinking water and after molting, the 3rd instars are exposed to the RFP strain present in the drinking water. In the GFP-Cm-RFP experiment, 2nd instar insects are infected with the GFP strain present in the drinking water; after 24 h, this drinking water is replaced by chloramphenicol-containing drinking water; the 3rd instars are then exposed to the RFP strain. In all infection schemes, the infection status was analyzed by dissection and microscopy in 3 dpi 2nd instar insects and in 3 dpi 3rd instar insects. b Infection status in 2nd instar insects. c Infection status in 3rd instar insects. In b, c, red and green fluorescence, DIC and merged images are shown. Note the “fluorescence” panels are merged images of red and green.