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. 2020 Mar 23;14(7):1627–1638. doi: 10.1038/s41396-020-0633-3

Fig. 6. Midgut closure requires passage through the constricted region and crypt colonization.

Fig. 6

a Infection scheme: 2nd instar insects were infected with different GFP-labeled strains and after molting, 3rd instars were infected with the wild-type RFP symbiont to verify the occurrence or not of midgut closure triggered by the first inoculation. b–j Infection status of 3 dpi 3rd instar insects, analyzed by dissection and microscopy, after infection in the 2nd instar with the wild type (b), the fliC::Tn5 (c), purL::Tn5 (d), ΔwaaF (e) and ΔphaB (f) mutants of B. insecticola, with E. coli (g) and C taiwanensis (h) that cannot pass through the CR, and with B. fungorum (i) and P. norimbergensis (j) that can pass through the CR and partially colonize the M4 crypts. In b–j, fluorescence images are shown. Note the fluorescence panels are merged images of red and green.