Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 19;11:3143. doi: 10.1038/s41467-020-16884-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a, b Top3β-KO mice displayed increased anxiety behavior in elevated maze test, as evidenced by reduced staying time (a) and number of entries in light arm (b) (18 mice/group). c Top3β-KO mice exhibited increased anxiety behavior in light-dark box test, as shown by fewer entries into the light box (11 mice/group). d, e Top3β-KO mice showed abnormal behavior in sociability test, as revealed by increased latency to initiate the first interaction with the other mouse in three-chamber sociability test (d), and increased nose-to-nose sniffing interaction in reciprocal social interaction test (e) (12 mice per group). f–h Top3β-KO mice displayed increased fear behaviors, as revealed by longer freezing time in training (f), context (g), and cue (h) test of the FC test (9 WT and 10 KO). The FC paradigm is in (Supplementary Fig. 1a). i, j Top3β-KO mice exhibited impaired ability to discriminate between a shock-paired context (A) and a neutral context (B). The paradigm is in Supplementary Fig. 1b. During training, Top3β-KO mice exhibited a significant longer freezing time when placed in context B, unlike the WT mice that showed similar short freezing time in both contexts (i). In the test day, Top3β-KO mice exhibited reduced discrimination index (j), as defined by a ratio in difference in freezing time between context A and B vs. the total freezing time. 11 WT and 12 KO mice were used. k, l Top3β-KO mice exhibited reduced learning and memory behaviors in water maze test, as revealed by longer time in finding the hidden platform (k), and explored longer average distance to the position of the platform (l) (7 mice/group). Two-tail Student t test was used for (a–h, j–l). Two-way ANOVA test was used for (i) in statistical analysis. P-value (a) 0.0414, (b) 0.0002, (c) 0.0149, (d) 0.0217, (e) 0.0369, (f) 0.0416, (g) 0.0131, (h) 0.0282, (i) 0.0136, (j) 0.0323, (k) 0.0455, (l) 0.0389. P-values < 0.05, 0.01, and 0.001 are marked as: *, **, ***, respectively. Data are presented as mean values ± SEM. Source data are provided as a Source Data file.