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. 2020 Jun 19;11:3135. doi: 10.1038/s41467-020-16872-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a PhTAC125 growth curves across all replicates in the 19 AA medium with one of them being ¹³C labelled. Each colour indicates which was the ¹³C-labelled amino acids for each replicate. Error bars represent SD of four different cell cultures. b Extracellular concentration of all the amino acids in time. Colour codes as in a. c PCA on the ¹³C spectra. Colour codes as in a. Grey shaded area includes the 95% confidence of the linear regression (coloured) line over the concentrations of the amino acids belonging to the same group. d Central PhTAC125 metabolic network, highlighting the possible entry points of the labelled amino acids. Circles close to compounds indicate the ¹³C signal recovered at the different time points (T3–T24) and coming from different amino acids (colour codes as in a) corresponding. Source data are provided as a Source Data file.