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. 2020 Jun 19;10:9962. doi: 10.1038/s41598-020-66997-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of deletion of methylcitrate synthase genes CIT2 and CIT3 on 3-ethylphenol (A) and 3-methylphenol (B) formation with or without supplementation of external propionate and on propionate consumption (C). CEN.PK2-1C expressing the 3-methylphenol pathway (JHY162) (^PpoptMSAS, ^optnpgA and ^optpatG¹⁴) and with the Δcit2Δcit3 double deletion (JHY197) were utilized for high-OD fermentations (starting OD = 5.0) at 30 °C in KP_i buffered YPD medium (pH 6.5) with or without supplementation of 10 mM propionate. Propionate consumption was followed in S. cerevisiae wild-type strain CEN.PK2-1C and deletion strains that either had peroxisomal (Δcit2), mitochondrial (Δcit3) or both methylcitrate synthases (Δcit2/Δcit3) deleted and were cultured (starting OD = 4) at 30 °C in KP_i buffered YPD medium (pH 6.5) supplemented with 10 mM propionate. Culture supernatants were analysed via HPLC for 3-alkylphenol production and propionate. Error bars represent standard deviations of biological duplicates.