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. 2020 Jun 19;10:9962. doi: 10.1038/s41598-020-66997-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Influence of overexpression of a propionyl-CoA synthetase on 3-ethylphenol (A) and 3-methylphenol (B) formation with and without supplementation of external propionate. Yeast strains CEN.PK2-1C expressing the 3-methylphenol pathway (^PpoptMSAS, ^optnpgA and ^optpatG¹⁴) and additionally the propionyl-CoA synthase ^optprpE, with or without the Δcit2Δcit3 double deletion (strains JHY185 and JHY218, respectively), were inoculated at an OD of 5 and cultivated for 144 h in KP_i buffered YPD medium (pH 6.5) with or without supplementation of 10 mM propionate. Culture supernatants were analysed via HPLC for 3-alkylphenol production. Error bars represent standard deviations of biological duplicates.