Figure 4.

3-Propylphenol formation via ‘reverse ß-oxidation’. 3-Propylphenol (A) and 3-methylphenol production (B) was measured in culture supernatants of CEN.PK2-1C expressing the 3-methylphenol pathway (^PpoptMSAS, ^optnpgA and ^optpatG¹⁴) with or without additional expression of the ‘reverse ß-oxidation’ pathway (ERG10, ^opthbd, ^optcrt and ^optter^21,22) (strains 194 and 162, respectively), and with additional pox1 deletion (strains JHY212 and JHY211, respectively). High-OD fermentations (starting OD = 5) were performed in biological duplicates at 30 °C in KP_i buffered YPD medium at pH 6.5. Culture supernatants were analysed via HPLC for 3-alkylphenol production. Error bars represent standard deviations.