. 2020 Jun 19;10:9962. doi: 10.1038/s41598-020-66997-5

Table 1.

Plasmids and yeast strains used in this study. Genes from Aspergillus nidulans (An), Aspergillus clavatus (Ac), Clostridium acetobutylicum (Ca), Penicillium patulum (Pp), Saccharomyces cerevisiae (Sc), Salmonella enterica serovar typhimurium (St), Treponema denticola (Td) and codon-optimized genes (opt) are indicated by prefixes in superscript.

Plasmid	Plasmid based on	Relevant features	Reference
pUG6-H	—	pBR322, hphNT1, Amp^r	²³
pRS42K	—	2 µ, kanMX, Amp^r	²⁴
pRS72N	—	2 µ, natMX, Amp^r	²⁴
pRCC-K	—	2 µ, kanMX, Amp^r, pROX3-^optCas9-tCYC1, pSNR52-gRNA	²⁵
pRS42K_prpE^783Δ	pJHV1	2 µ, kanMX, Amp^r, pMET25, tCYC1, pTDH3-^StoptprpE^G783-tPGK1	This work
pJHV19	pRCC-K	2 µ, kanMX, Amp^r, pROX3-^optCas9-tCYC1, pSNR52-gRNA for SFA1	This work
pJHV54	pRCC-K	2 µ, kanMX, Amp^r, pROX3-^optCas9-tCYC1, pSNR52-gRNA for CIT3	This work
pJHV62	—	ColE1, Amp^R,ConL3′-pTDH3-^Tdoptter-tADH1 –ConRE‘	This work
pJHV65	—	ConLS‘-pPGK1p-^ScERG10-tVMA16 -ConR1′-ConL1′-pCCW12-^Caopthbd-tIDP –ConR2′-ConL2′-pENO2-^Caoptcrt-tPGK1 –ConR3′-ConL3′-pTDH3-^Tdoptter-tADH1 –ConRE‘-natMX-LEU2 3′Hom-KanR-ColE1-LEU 5′Hom	This work
pRS72N_ADY2	—	2 µ, natMX, Amp^r, pHXT7^-1–392-^ScADY2-tCYC1	²⁶
pRS72N_JEN1	—	2 µ, natMX, Amp^r, pHXT7^-1–392-^ScJEN1-tCYC1	²⁶
SiHV110	—	ConLS’-gfp-dropout-ConRE’-natMX-LEU2 3’Hom-KanR-ColE1-LEU 5’Hom	This work (provided by Simon Harth)
pYTK3.41	—	ColE1, Cam^R, ^Tdoptter	This work (provided by Fernando Garcés Daza)
pYTK3.43	—	ColE1, Cam^R, ^Caoptcrt	This work (provided by Fernando Garcés Daza)
pYTK3.47	—	ColE1, Cam^R, ^ScERG10	This work (provided by Fernando Garcés Daza)
pYTK3.49	—	ColE1, Cam^R, ^Caopthbd	This work (provided by Fernando Garcés Daza)
pYTK_Erg10	—	ColE1, Amp^R,ConLS‘-pPGK1-^ScERG10-tVMA16 -ConR1‘	This work (provided by Fernando Garcés Daza)
pYTK_Hbd	—	ColE1, Amp^R,ConL1′-pCCW12-^Caopthbd-tIDP –ConR2′	This work (provided by Fernando Garcés Daza)
pYTK_Crt	—	ColE1, Amp^R,ConL2′-pENO2-^Caoptcrt-tPGK1 –ConR3′	This work (provided by Fernando Garcés Daza)
pYTK01	—	ColE1, Cam^R, gfp-dropout	²⁷
pYTK05	—	ColE1, Cam^R, ConL3	²⁷
pYTK09	—	ColE1, Cam^R, TDH3p	²⁷
pYTK53	—	ColE1, Cam^R, ADH1t	²⁷
pYTK72	—	ColE1, Cam^R, ConRE	²⁷
pYTK95	—	ColE1, Cam^R, Amp^R-ColE1	²⁷
pVS06	—	CEN6ARS4, kanMX, Amp^r, pHXT7^-1–392-^ScERG10-tVMA16, pPGK1-^Caopthbd-tEFM1, pTPI1-^Caoptcrt-tYHI9, pPYK1-^Tdoptter-tIDP1, pADH1-^CaoptadhE2-tRPL3, pTDH3-^EcopteutE-tRPL41B	²²
pRS62H_ter	—	2 µ, natMX, Amp^r, pHXT7^-1–392-^Tdoptter-tFBA1	²¹
pAB02	—	2 µ, natMX, Amp^r, pROX3-^optCas9-tCYC1, pSNR52-gRNA for POX1	This work (provided by Alexander Bissl)
pAB09	—	2 µ, natMX, Amp^r, pROX3-^optCas9-tCYC1, pSNR52-gRNA for CIT2	This work (provided by Alexander Bissl)
*S. cerevisiae* strain	Parent strain	Relevant features	Reference
CEN.PK2-1C	—	MATa leu2-3,112 ura3-52 trp1-289 his3-Δ1 MAL2–8^c SUC2	²⁸
JHY65	CEN.PK2-1C	psfa1-sfa1Δ::pTDH3-^StoptprpE-tSFA1	This work
JHY162	CEN.PK2-1C	ura3::pPGK1-^PpoptMSAS-tCYC1, pHXT7^-1–392- ^AnoptnpgA-tFBA1, pFBA1-^AcoptpatG-tADH1	¹⁴
JHY164	CEN.PK2-1C	cit2Δ	This work
JHY174	JHY164	cit2Δ cit3Δ	This work
JHY175	CEN.PK2-1C	cit3Δ	This work
JHY179	JHY65	psfa1-sfa1Δ::pTDH3-^StoptprpE-tSFA1 cit3Δ	This work
JHY180	JHY179	psfa1-sfa1Δ::pTDH3-^StoptprpE-tSFA1 cit3Δ cit2Δ	This work
JHY185	JHY180	psfa1-sfa1Δ::pTDH3-^StoptprpE-tSFA1 cit3Δ cit2Δ ura3::pPGK1-^PpoptMSAS-tCYC1, pHXT7^-1–392- ^AnoptnpgA-tFBA1, pFBA1-^AcoptpatG-tADH1	This work
JHY194	JHY162	ura3::pPGK1-^PpoptMSAS-tCYC1, pHXT7^-1–392- ^AnoptnpgA-tFBA1, pFBA1-^AcoptpatG-tADH1 leu2::pPGK1-^ScERG10-tVMA16, pCCW12-^Caopthbd-tIDP, pENO2-^Caoptcrt-tPGK1, pTDH3-^Tdoptter-tADH1, pTEF-natMX-tTEF	This work
JHY196	CEN.PK2-1C	pox1Δ	This work
JHY197	JHY174	cit2Δ cit3Δ ura3::pPGK1-^PpoptMSAS-tCYC1, pHXT7^-1–392- ^AnoptnpgA-tFBA1, pFBA1-^AcoptpatG-tADH1	This work
JHY211	JHY196	pox1Δ ura3::pPGK1-^PpoptMSAS-tCYC1, pHXT7^-1–392- ^AnoptnpgA-tFBA1, pFBA1-^AcoptpatG-tADH1	This work
JHY212	JHY211	pox1Δ ura3::pPGK1-^PpoptMSAS-tCYC1, pHXT7^-1–392- ^AnoptnpgA-tFBA1, pFBA1-^AcoptpatG-tADH1 leu2::pPGK1-^ScERG10-tVMA16, pCCW12-^Caopthbd-tIDP, pENO2-^Caoptcrt-tPGK1, pTDH3-^Tdoptter-tADH1, pTEF-natMX-tTEF	This work
JHY218	JHY65	sfa1p-sfa1Δ::TDH3p-^StoptprpE-SFA1t ura3::pPGK1-^PpoptMSAS-tCYC1, pHXT7^-1–392- ^AnoptnpgA-tFBA1, pFBA1-^AcoptpatG-tADH1	This work
JHY229	JHY185	psfa1-sfa1Δ::pTDH3-^StoptprpE-tSFA1 cit3Δ cit2Δ ura3::pPGK1-^PpoptMSAS-tCYC1, pHXT7^-1–392- ^AnoptnpgA-tFBA1, pFBA1-^AcoptpatG-tADH1 pacs2-acs2Δ::pTEF-hphNT1-tCYC1	This work

Other abbreviations: hphNT1: hygromycin resistance; Amp^r: ampicillin resistance; Cam^R: chloramphenicol resistance; Kan^R: kanamycin resistance; kanMX: geneticin resistance; natMX: clonat resistance. If not stated otherwise, promoters (p) were taken 1-500 bp upstream and terminators (t) 1-300 bp downstream of respective open reading frames.