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. 2020 Jun 19;11:3140. doi: 10.1038/s41467-020-16907-0

Fig. 8. Distribution of MeCP2, H3K27me3 and H3K9ac.

Fig. 8

a–c Distribution of MeCP2, H3K27me3 and H3K9ac within differentially expressed genes between WT and Mecp2 KO. N = 3 (per genotype, WT vs Mecp2 KO) from biologically independent animals. MeCP2 (a), H3K27me3 (b), and H3K9ac (c) distribution in wild-type chromatin plotted 1 kb up- and 10 kb downstream of the TSS of genes that are up- (purple, n = 890) or down-regulated (green, n = 699) in Mecp2 KO. N = 2 (WT) from biologically independent animals. For MeCP2 (a), p = 6.62 × 10−30 (Up, TSS vs proximal regions); p = 4.13 × 10−7 (Up, Gene body vs proximal regions); p = 0.05 (Down, TSS vs proximal regions); p = 0.02 (Down, Gene body vs proximal regions). For H3K27me3 (b), p = 1.79 × 10−43 (Up, TSS vs proximal regions); p = 2.02 × 10−6 (Up, Gene body vs proximal regions); p = 2.53 × 10−5 (Down, TSS vs proximal regions); p = 0.26 (Down, Gene body vs proximal regions). For H3K9ac (c), p = 2.90 × 10−32 (Up, TSS vs proximal regions); p = 0.17 (Up, Gene body vs proximal regions); p = 1.88 × 10−6 (Down, TSS vs proximal regions); p = 3.21 × 10−4 (Down, Gene body vs proximal regions). (two-tailed Mann–Whitney test, ***p < 0.0001). Box-and-whisker plots show median, 10–90 percentile, and min and max values. The shaded areas are up to ±S.D. from the average profile (a–c). d Schematic diagram of MeCP2 chromatin interaction and its function in gene expression regulation.