Fig. 1. MA inhibits heterologously expressed mouse PIEZO2 currents in N2A^{Piezo1−/−} cells.

a Representative whole-cell patch-clamp recordings elicited by mechanical stimulation (at −60 mV) of control and margaric acid (MA)-treated N2A^{Piezo1−/−} cells transfected with Piezo2 variant 2 (V2). b Normalized current densities elicited by maximum displacement of MA-treated N2A^{Piezo1−/−} cells transfected with Piezo2 V2. A Boltzmann function, Eq. (2), was fitted to the data (IC₅₀ = 190.6 ± 14.7 SEM). Circles are mean ± SD. n is denoted above the x-axis of c. c Displacement thresholds required to elicit PIEZO2 V2 currents of control and N2A^{Piezo1−/−} cells. Boxplots show mean (square), median (bisecting line), bounds of box (75^th to 25^th percentiles), outlier range with 1.5 coefficient (whiskers), and minimum and maximum data points. n is denoted above the x-axis. Two-tailed unpaired t-test. d Representative PIEZO2 currents (at −60 mV) of control and MA (50 µM each day for 4 days)-treated N2A^{Piezo1−/−} cells transfected with Piezo2 V2. e PIEZO2 V2 current densities elicited by maximum displacement of control and MA (50 µM for 18 h and each day for 4 days)-treated N2A^{Piezo1−/−} cells. Error bars represent SD. n is denoted above the x-axis. Two-tailed unpaired t-test. f Displacement thresholds required to elicit PIEZO2 V2 currents of control and MA (50 µM each day for 4 days)-treated N2A^{Piezo1−/−} cells transfected with PIEZO2 V2. Boxplots show mean (square), median (bisecting line), bounds of box (75^th to 25^th percentiles), outlier range with 1.5 coefficient (whiskers), and minimum and maximum data points. n is denoted above the x-axis. Two-tailed unpaired t-test. p-values are denoted above the boxes and bars.