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. 2020 Jun 19;11:2997. doi: 10.1038/s41467-020-16816-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Normalized current density elicited by maximum displacement of MA-supplemented N2A cells (expressing endogenous Piezo1; triangles) and MA-supplemented N2A^{Piezo1−/−} cells transfected with Piezo2 (circles). A Boltzmann function, Eq. (2), was fitted to the data. Symbols are mean ± SD. From left to right, n for PIEZO1: 6, 4, 4, 5, 13, and 7 and PIEZO2: 5, 5, 5, 6, 9, 9, 6, and 7. b Normalized current densities elicited by maximum displacement of MA-supplemented N2APiezo1^−/− cells transfected with Piezo2, treated with and without latrunculin A (red and black curves, respectively). A Boltzmann function, Eq. (2), was fitted to the data. Circles are mean ± SD. From left to right, n for PIEZO2 with latrunculin A: 6, 5, 7, 5, 8, 7, 4, and 5, and PIEZO2: 5, 5, 5, 6, 9, 9, 6, and 7. c Normalized current densities elicited by maximum displacement of MA-supplemented N2A cells (expressing endogenous Piezo1), treated with and without latrunculin A (red and black curves, respectively). A Boltzmann function, Eq. (2), was fitted to the data. Triangles are mean ± SD. From left to right, n for PIEZO1 with latrunculin A: 8, 5, 6, 6, 6, and 5, and PIEZO1: 6, 4, 4, 5, 13, and 7. d Top, ribbon representation of PIEZO2 monomer (PDB ID: 6KG7; gray) highlighting the residues that were exchanged for those of PIEZO1 (yellow). Bottom, ribbon representation of PIEZO1 monomer (PDB ID: 5Z10; gray) highlighting the residues that were exchanged for those of PIEZO2 (blue). e Inhibition by MA supplementation of N2A cells and N2A^{Piezo1−/−} cells transfected with Piezo2, and Piezo2-Piezo1 and Piezo1-Piezo2 beam chimeras. Error bars represent SD. n is denoted above the x-axis. Two-tailed unpaired t-test. f Normalized current densities elicited by maximum displacement of MA-supplemented N2A cells (expressing endogenous Piezo1) and N2A^{Piezo1−/−} cells transfected with Piezo2, and Piezo2-Piezo1 and Piezo1-Piezo2 beam chimeras treated with and without latrunculin A. Error bars represent SD. n is denoted above the x-axis. Two-tailed unpaired t-test for PIEZO1 and two-tailed Mann–Whitney test for PIEZO2, and PIEZO2–PIEZO1 and PIEZO1–PIEZO2 beam chimeras. p-values are denoted above the bars.