Fig. 4. MA decreases action potentials elicited by mechanical stimuli in mouse DRG neurons.
a Representative current-clamp recordings of membrane potential changes elicited by mechanical stimulation in control and MA (300 µM)-treated mouse DRG neurons (up to 10 and 15 µm indentation, respectively). b Membrane potential peak vs. mechanical indentation of independent control (black; n = 10) and MA-treated (red; n = 7) mouse DRG neurons. Top panel shows the displacement threshold required to elicit an action potential in these neurons. Boxplots show mean (square), median (bisecting line), bounds of box (75th to 25th percentiles), outlier range with 1.5 coefficient (whiskers), and minimum and maximum data points. Two-tailed unpaired t-test. p-value is denoted on the right. c Representative current-clamp recordings of membrane potential changes elicited by a 1 Hz mechanical stimulus train of control (black) and MA-treated (red) mouse DRG neurons. Inset illustrates the progressive decrease in membrane potential as the pulse number progresses. d Left: number of action potentials elicited per sweep vs. mechanical indentation of control (black; n = 6) and MA-treated (red; n = 6) mouse DRG neurons. Right: raster plots displaying the action potentials elicited by 12 µm of indentation. Bars indicate stimuli that elicited action potentials. Columns and rows represent cells and sweeps, respectively.