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. 2020 Jun 19;10:9964. doi: 10.1038/s41598-020-66472-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Concentration of essential elements in dentine and their relationship with age. (a) Concentration of essential elements in all the teeth (b,c). Concentration of essential elements in men and women teeth (d,e). Concentration of essential elements in the different types of teeth. Each point represents the mean concentration value of each trace element. Comparisons between the different age ranges (<30, 30–50, >50 years) were made by the Kruskal-Wallis test. P < 0.05 was statistically significant. *p < 0.05; **p < 0.01; ***p < 0.001. This figure has been generated using GraphPad Prism 5 software.